How to calculate primer annealing temperature? - annealing time calculation for primers
As the primer annealing temperature to calculate? Please go ahead and the appropriate form (manually)? In my experience with the DNA template and primer base pairs 18 to 25 base pairs (minimum size = 18 first and height of the first maximum is 25). thank you reply!
Sunday, December 6, 2009
Annealing Time Calculation For Primers How To Calculate Primer Annealing Temperature?
1 comment:
64.9 Tm = 41 * (YG + ZC-16.4) / (WA XT + + and G + ZC)
where w, x, y, z are the number of bases A, T, G, C in the sequence, respectively.
Shamelessly lifted from the link below (you even have a simulator built in so you can check your answer!)
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